Catalase | Definition, Function & Importance
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The catalase test is a quick and easy method for the differentiation of Streptococcus and Staphylococcus. All Streptococcus species are catalase negative and Staphylococcus are catalase positive (bubble production) The medium used should NOT have blood incorporated since erythrocytes have catalase activity, which can give false positive results. The test should be done on young cultures 18-24 hours. The reagent used is 3% hydrogen peroxide | Medical Lab Technologist
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8.6: Catalase Activity (Demonstration)
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libretexts.org
The catalase test tests for the presence of catalase, an enzyme that breaks down the harmful substance hydrogen peroxide into water and oxygen. If an organism can produce catalase, it will produce bubbles of oxygen when hydrogen peroxide is added to it Purpose of Catalase Test The morphologically similar Enterococcusor Streptococcus (catalase negative) and Staphylococcus (catalase positive) can be differentiated using the catalase test. Also valuable in differentiating aerobic and obligate anaer
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